Journal: BMC Cancer

Article Title: Presence of S100A9-positive inflammatory cells in cancer tissues correlates with an early stage cancer and a better prognosis in patients with gastric cancer

doi: 10.1186/1471-2407-12-316

Figure Lengend Snippet: Expression of S100A9 in gastric cancer and adjacent non-cancerous tissues. ( A ) Different expression value of S100A9 in 72 gastric cancer tissues and paired no-cancerous tissues by analyzing data from illumina Sentrix BeadChip cDNA microarray. (B-E) Immunohistochemical staining of S100A9 in gastric cancer tissues ( B ) metastatic lymph nodes ( C ), chronic gastritis ( D ), and adjacent non-cancerous gastric mucosa ( E ). S100A9 localization was revealed as brown or red granulated loci in the cytoplasm of infiltrating inflammatory cells, especially in mononuclear phagocytes and neutrophil granulocytes. (magnification 200×).

Article Snippet: Expression of S100A9, S100A8 and S100A8/A9 were also detected in Cybrdi tissue microarray slides (IC00-01-001, Cybrdi, Xi'an, China) containing chronic gastritis with metaplasia (57 cases) and gastric carcinoma tissues (23 cases).

Techniques: Expressing, Microarray, Immunohistochemical staining, Staining

Journal: BMC Cancer

Article Title: Presence of S100A9-positive inflammatory cells in cancer tissues correlates with an early stage cancer and a better prognosis in patients with gastric cancer

doi: 10.1186/1471-2407-12-316

Figure Lengend Snippet: Immunofluorescence images of S100A9, S100A8 and S100A8/A9 proteins in tissue microarray slides containing gastric cancer tissues (A-J) and chronic gastritis tissues (K-T), and chronic appendicitis tissues with exacerbation (U-Y). S100A9 and S100A8 were detected by monoclonal antibody, prelabeled with the Zenon Alexa Fluor Mouse IgG Labeling Kit (with green and red fluorescence respectively). The nucleus was stained by DAPI. S100A8/A9 heterodimers were detectable using the dimer-specific antibody 27E10 from BMA Biomedicals prelabeled with green fluorescence. The co-localization of S100A9 and S100A8 or S100A8/A9 was showed in merged pictures ( D , I , N , S , X ) and larger merged pictures ( E , J , O , T , Y ). White arrow in 3 T shows co-localization of S100A9 and S100A8/A9 in chronic gastritis. Bar length, 50 μm.

Article Snippet: Expression of S100A9, S100A8 and S100A8/A9 were also detected in Cybrdi tissue microarray slides (IC00-01-001, Cybrdi, Xi'an, China) containing chronic gastritis with metaplasia (57 cases) and gastric carcinoma tissues (23 cases).

Techniques: Immunofluorescence, Microarray, Labeling, Fluorescence, Staining

Journal: FEBS Open Bio

Article Title: PRDM 14 is overexpressed in chronic pancreatitis prior to pancreatic cancer

doi: 10.1002/2211-5463.12519

Figure Lengend Snippet: Expression of PRDM 14 in pancreatitis. (A) Representative IHC images for PRDM 14 in acute and chronic pancreatitis in TMA slide. Scale bars in image, 50 μm. (B) Staining scores and P values for PRDM 14 in tissues, 13 normal pancreas, 115 PDAC , 36 cancer adjacent, 30 Pan IN , six acute pancreatitis, and 58 chronic pancreatitis. Error bars in graphs represent mean ± SD . Student's t ‐test: ** P < 0.01, ns, not significant.

Article Snippet: Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011).

Techniques: Expressing, Staining

Journal: FEBS Open Bio

Article Title: PRDM 14 is overexpressed in chronic pancreatitis prior to pancreatic cancer

doi: 10.1002/2211-5463.12519

Figure Lengend Snippet: Pancreatitis mouse models. Acute and chronic pancreatitis was induced by intraperitoneal cerulein injection. Saline was used as control. (A) Representative images of the pancreases stained with H&E or Sirius red, and IHC for PRDM 14 are shown. Scale bars in image, 200 μm. (B) High‐power magnification of IHC for PRDM 14 of pancreas in chronic pancreatitis mouse models. The left panel is the enlarged image of the black box in the lower right panel in A. Scale bars in image, 50 μm.

Article Snippet: Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011).

Techniques: Injection, Saline, Control, Staining

Journal: FEBS Open Bio

Article Title: PRDM 14 is overexpressed in chronic pancreatitis prior to pancreatic cancer

doi: 10.1002/2211-5463.12519

Figure Lengend Snippet: Pancreases with spleen of pancreatitis mouse models. Image of all pancreases with spleen of acute pancreatitis and chronic pancreatitis mice ( n = 6 per group).

Article Snippet: Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011).

Techniques: